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Seminar: Immunoaffinity Capillary Electrophoresis as a Tool for the Isolation
Start Date: 1/9/2014Start Time: 4:30 PM
End Date: 1/14/2014End Time: 6:00 PM

Event Description
Norberto A. Guzman, PhD, MSc, Bioanalysis, Toxicoproteomics, Immunochemistry, Princeton Biochemicals Inc.

 

"Immunoaffinity Capillary Electrophoresis as a Tool for the Isolation and Characterization of Biomolecules in Simple and Complex Mixtures. Application to Biomarkers of Inflammatory Processes."

Proteomic technologies represent useful strategies towards high-throughput, simultaneous analysis of hundreds or even thousands of proteins and peptides leading to the discovery of biomarkers for early diagnosis and prognosis of inflammatory diseases. It has been shown that increased levels of numerous proteins and peptides have been associated with certain inflammatory processes. In most instances chronic inflammatory diseases are very complex and it might require a panel of multiple biomarkers in order to achieve sufficient clinical efficacy.

Recent evidence suggests that each patient affected by an inflammatory disease may have a unique subset of molecular patho-genetic/proteomic derangements, and the biomarkers may circulate in biological fluids in a number of distinct molecular forms. The labor-intensive task of isolating and characterizing individual modified proteins must continue, especially given the expanding list of unknown co- and post-translational modifications.

Immunoaffinity capillary electrophoresis (IACE) is a technology that is becoming a powerful tool as a multi-dimensional platform for the rapid and effective isolation and characterization of a panel of biomarkers. The constant evolution of technologies makes multi-dimensional IACE strategy a crucial player in clinic and basic comprehensive proteomics.

IACE combines the use of antibodies, and/or other affinity ligands, as highly selective capture agents with the high resolving power of capillary electrophoresis. This two-dimensional hybrid technology permits the quantification and characterization of several protein biomarkers simultaneously, including subtle structural changes such as variants, isoforms, peptide fragments, and co-/post-translational modifications. Furthermore, the results are rapid, sensitive, can be performed at a relative low cost, without the introduction of false positive or false negative data.

In this presentation, I will discuss important aspects of the technology of IACE and its use in the characterization of biomarkers of inflammation. Furthermore, I will review future directions for this rapidly evolving field of proteomics that can aid in diagnosing disease, estimating prognosis, and monitoring treatment. Protein modifications can change a protein’s physical or chemical properties, conformation, activity, cellular localization, stability, and interactions.

References:
1. Guzman NA, Phillips TM. Electrophoresis 32 (13), 1565-1578 (2011).
2. Guzman NA. American Laboratory 40 (20), 18-29 (2008).
3. Guzman NA, Blanc T, Phillips TM. Electrophoresis 29 (16), 3259-3278 (2008).
4. Guzman NA, Stubbs RJ, Phillips TM. Drug Discovery Today: Technologies 3 (1), 29-37 (2006).
5. Guzman NA, Phillips TM. Analytical Chemistry 77 (3) (A-Page), 60A-67A (2005).
6. Guzman NA. Analytical Bioanalytical Chemistry 378 (1), 37-39 (2004)
7. Guzman NA. Electrophoresis 24 (21), 3718-3727 (2003).
8. Guzman NA, Stubbs RJ. Electrophoresis 22 (17), 3602-3628 (2001).
9. Guzman NA. Prolyl 4-Hydroxylase and Other Structurally-Related Proteins, Chapter 1: 1-64, Editor: Guzman NA. New York, New York: Marcel Dekker, Inc./CRC Press (1998).
Location:
Disque Hall, Room 109, 32 South 32nd Street, Philadelphia, PA 19135
Audience:
  • Alumni
  • Faculty
  • Parents & Families
  • Prospective Students
  • Public
  • Staff

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