| Start Date: | 10/12/2016 | Start Time: | 4:00 PM |
| End Date: | 10/12/2016 | End Time: | 5:30 PM |
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Event Description
BIOMED Seminar
Title:
Control of Glucose Metabolism for Tendon Repair
Speaker:
Motomi Enomoto-Iwamoto, DDS, PhD
Research Associate Professor
Division of Orthopedic Surgery
Department of Surgery
Children’s Hospital of Philadelphia (CHOP)
University of Pennsylvania School of Medicine
Abstract:
Incomplete tendon healing leads to significant mobility restriction, pain, and substantial health care costs. To develop novel targeted therapies for tendon injury, it is necessary to define the molecular and cellular changes and mechanisms governing the tendon healing process. We have been studying tendon progenitors appearing in injured tendons using the mouse Achilles tendon injury model. Cells recruited to injured tendons (inTPCs) have progenitor characteristics and are involved in chondroid degeneration and tendon regeneration. These cell populations have stronger chondrogenic potential compared to the progenitors, suggesting that tendon progenitors are isolated from normal, uninjured tendons, thereby indicating that the newly-identified inTPCs may be important to understanding the tendon healing process.
Up-regulation Inflammatory cytokines are up-regulated in injured tendons. Interestingly, IL-1β inhibits tenogenic differentiation of inTPCs and increases their lactate synthesis, and this inhibition of lactate synthesis competes against the IL-1β action on tenogenic differentiation, while glycolysis and lactate synthesis occurs in wound, inflammation and cancer. Therefore, we analyzed activities of glycolysis and lactate synthesis in injured tendons with 13C-glucose labeling. The molar percent enrichment of 13C-latate was strongly increased at one week post-injury and remained high after four weeks. Furthermore, dichloroacetat (DCA), an inhibitor of lactate synthesis, improved recovery of collagen fiber formation and biomechanical properties in the mouse Achilles tendon injury model. The findings indicate that glycolysis and lactate synthesis can be novel therapeutic targets for tendon repair.
For more info, please visit drexel.edu/biomed.
Biosketch:
Dr. Enomoto-Iwamoto received a Doctorate in Dental Medicine and a PhD from Osaka University in Japan. Following a postdoctoral fellowship at the University of Pennsylvania, she was appointed Assistant Professor with tenure at Osaka University. In 2002, she was appointed as an Associate Professor at Thomas Jefferson University and joined the Department of Orthopaedic Surgery. In 2011, she joined the Department of Surgery, Orthopaedic Division at the Children's Hospital of Philadelphia (CHOP). She maintained a high research activity in the field of musculoskeletal medical science and continued to publish over 75 original articles in high impact journals. She has also contributed to international, domestic, and local academic societies, serving as an editorial board member, grant/manuscript reviewer, and as co-organizer of a local academic seminar series. Her research interests include molecular mechanisms that control endochondral ossification and articular cartilage development and organization, as well as tendon biology and pathology. |
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Location:
Papadakis Integrated Sciences Building (PISB), Room 120, located on the northeast corner of 33rd and Chestnut Streets. |
Audience:
Undergraduate StudentsGraduate StudentsFacultyStaff |
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