Drexel University - Comprehensive, integrated academics enhanced by co-operative education, technology, and research opportunities. | Drexel University
Drexel University
Search events. View events.

All Categories

Click for help in using calendar displays. Print the contents of the current screen.
Display Format: 
Event Details
Notify me if this event changes.Add this event to my personal calendar.
Go Back
Physics Colloquium: Structural Dynamics by Single Molecule Biophysical Approaches: Polarized...
Start Date: 10/10/2019Start Time: 3:30 PM
End Date: 10/10/2019End Time: 4:30 PM

Event Description

Yale E. Goldman, MD, PhD, University of Pennsylvania


Structural Dynamics by Single Molecule Biophysical Approaches: Polarized Fluorescence Microscopy and High-Speed Laser Tweezers


Molecular motors are ‘smart’ protein nanomachines that enable muscle and heart contraction and carry essential intracellular cargos to specific destinations within each cell. Myosin is the molecular motor that travels along intracellular actin cytoskeletal filaments to perform these tasks. A relatively new approach to studying cell biological systems, termed single molecule biophysics, makes use of novel instrumentation and quantitative analysis to obtain details not available from classical biochemical methods on millions of molecules. Taking an average always loses information about the true distribution among individuals! Our single molecule experiments on the myosin V motor showed how it walks along actin filaments “hand-over-hand” to avoid “losing track”. It can’t derive energy from its chaotic environment, but nevertheless, it harnesses local fluctuations usefully. The linkage between elementary steps in the enzymatic cycle of myosin motors and transduction of chemical energy into mechanical work has been controversial for many years. Novel methods using the infrared laser trap, also called laser tweezers, improve temporal resolution some 50-fold, finally showing the sequence of biochemical and mechanical reaction steps. Technology to achieve these improvements, quantitative analysis of the noisy traces, and the conclusions about the mechanism will be explained.

Contact Information:
Name: Professor Frank Ferrone
Email: ferronfa@drexel.edu
Disque Hall, Room 919, 32 South 32nd Street, Philadelphia, PA 19104
  • Undergraduate Students
  • Graduate Students
  • Faculty

  • Display Month:

    Advanced Search (New Search)
    Date Range:
    Time Range:

    Special Features: 

    Select item(s) to Search

    Select item(s) to Search
    Select item(s) to Search
    Select item(s) to Search